The collection and preservation of ELISA specimens are ready for you! -Huaqiang Electronic Network

Probe domestic double-head probe 038-BU-6.3L needle head shape is pointed and clawed

EL-C1600N100013-B

Spot Remarks 2017+

This morning, our manager of the company received an online consultation from Zhou Zhong, a student from Huazhong University of Science and Technology, saying that he had just been exposed to an ELISA experiment. He was not very familiar with many technical problems, and he was afraid that the guide would ask more questions and would like to consult us. . The first step in doing the experiment is of course to collect the sample and collect and save the sample. I hope we can help him. For the problem of Zhou’s classmates, Qian Manager made a detailed answer for it online.

The most commonly used clinical specimens for ELISA are serum (slurry), and sometimes saliva, cerebrospinal fluid, urine, feces, etc. are used for specific testing purposes. At present, the markers used in the clinical use of serum samples generally include antigens and antibodies of infectious pathogens, tumor markers, hormones, special proteins, cytokines and therapeutic drugs. For the collection of serum samples for the determination of hormones and therapeutic drugs, it should be noted that the collection time or even the body position may have an impact on the measurement results. If cortisone is between 4 and 6 in the morning, there will be a peak: growth hormone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) are released in a paroxysmal manner. Therefore, when measuring such hormones It is necessary to take several blood samples at closely spaced intervals, with the median value as the measured value. Another example is when the renin activity is significantly increased from the supine position to the standing position. Another example is the detection of therapeutic drugs, which should be selected according to the pharmacokinetics of the optimal time after taking the drug. The collection of serum samples for the detection of antigens and antibodies, tumor markers and specialty proteins of infectious pathogens has no time or positional effects, but the following aspects should be considered in terms of handling and preservation:

1, should pay attention to avoid serious hemolysis. Hemoglobin contains a heme group, which has a peroxide-like activity. Therefore, in an ELISA assay using HRP as a labeling enzyme, if the hemoglobin concentration in a serum sample is high, it is easily adsorbed during the incubation. In the solid phase, it reacts with the HRP substrate added later to develop color.

2, sample collection and serum separation should pay attention to avoid bacterial contamination, a bacterial growth, some of the enzymes secreted by it may decompose proteins such as antigens and antibodies; second, some bacterial endogenous enzymes such as the large intestine The beta-galactosidase of Bacillus itself produces non-specific interference with assays labeled with the corresponding enzymes.

3. If the serum sample is isolated by aseptic operation, it can be stored at 2~8 °C for one week. If it is operated by bacteria, it is recommended to freeze it. Long-term storage of the sample should be below -70 °C.

4. Frozen serum samples should be taken care of to avoid repeated freezing and thawing caused by power outages. The mechanical shear force generated by repeated freezing and thawing of the specimen will destroy the molecules such as proteins in the specimen, causing false negative results. In addition, the mixing of freeze-thaw specimens should also be noted, do not violently shake, and repeatedly invert and mix.

5. If the specimen is turbid or floc due to bacterial contamination during storage, the supernatant should be taken after centrifugation and sedimentation.

After reading our introduction, Mr. Zhou expressed his satisfaction and praised the service of our company. Later, we need our elisa kit to choose our home.

Using the Shanghai Huding ELISA kit for experiments, we can test for you for free, and the remaining reagents can be mailed to you after the results are available. This test service only charges for the kit, and all other auxiliary reagents are free.