UV1200 UV-visible spectrophotometer - Multi-component analysis method

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UV-1200 UV-Vis Spectrophotometer - Multi-component Analysis

Key words: UV-visible spectrophotometry; multi-component analysis; US analytical instrument ; UV-1100; UV-1200

$</p> At one wavelength, the total absorbance of a solution is equal to the sum of the absorbances of the individual components. From this point, the individual components of the mixture can be analyzed. If there is a mixture, although it is multi-component, the absorption bands of the components do not overlap. At a certain wavelength, the absorption of one component is large, while the other components have no absorption at this wavelength. Thus, the quantitative determination of this component can be carried out at this wavelength using the above-described standard curve method. If a multi-component mixture, the absorption bands overlap with each other, but can follow Beer's law, it can be quantified by the method of solving the joint agenda. If the mixture contains n known components, the molar absorption coefficients of the n components at n wavelengths are required to be performed at n appropriate wavelengths. The choice of wavelength should be such that the absorption coefficient of one component is greater than the absorption coefficient of the other components, so that higher accuracy can be obtained. If the absorption bands of several components in the mixture overlap each other and do not follow Beer's law, they cannot be resolved by the method of solving the cubic equation. At this time, it is difficult to quantify by spectrophotometry. Fry uses the calibration method to distribute the five groups in the mixed sample into different solutions, resulting in a series of calibration curves. On the basis of the calibration curve, the content of the five components was obtained by analysis. But the method is very cumbersome and inconvenient to use. It is more difficult if the mixture contains components of an unknown absorption band. At this time, it is best to separate and purify by chemical method. However, it can also be directly analyzed by spectrophotometry. Morton uses the change in the shape of the absorption spectrum with impurities to calculate the amount of cod liver oil when the mixture contains impurities. Although spectrophotometry can be used for multi-component analysis, the method is cumbersome. In recent years, many spectrophotometers have multi-component analytical accessories. It is very simple, rapid and accurate to analyze and calculate the content of various components in a mixture by means of a microprocessor. This allows the spectrophotometric method to play a greater role in the quantitative analysis of the mixture. Similarly, it can also be quantified by the derivative spectroscopy described later. Key words: UV-visible spectrophotometry; multi-component analysis; US-analytical instrument ; UV-1100; UV-1200</p> </div> </div> <div class="tech-detail -share">  <div class="bdsharebuttonbox"> <a href="#" class="bds_qzone" data-cmd="qzone" title="Share to QQ Space"> </a> <a href="#" class="bds_tsina" data-cmd="tsina" title="Share to Sina Weibo"></a> <a href="#" class="bds_weixin" data -cmd="weixin" title="Share to WeChat"></a> <span>Share to:</span> </div> <script>window._bd_share_config = { "common": { "bdSnsKey": { }, "bdText": "", "bdMini": "1", "bdMiniList": false, "bdPic": "", "bdStyle": "2", "bdSize": "16" }, "share" : {} }; with (document) 0[(getElementsByTagName($

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