Huding ELISA kit detection program sharing - Database & Sql Blog Articles

Test - uppercase JPG - memory 146K

Kaixin micro test

Test probe P100-M3

EL-C1600N100013-B

Test and test work has become an important means and method to measure and reflect the quality of elisa kit. Only test and test work

Only after careful attention can we effectively improve and guarantee the quality of the experimental results of the ELISA kit. Hu Ding is here to share with you the specific experimental procedure of the ELISA kit.
The specific steps are as follows:

1. Loading: Add 100 ul of standard or test sample (activated) to each well, mix the reaction plate thoroughly and set at 37 ° C for 120 minutes.

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2. Washing the plate: The reaction plate was thoroughly washed 4-6 times with a washing solution, and dried on a filter paper.
3. Add 100 ul of the first antibody working solution to each well. The reaction plate was thoroughly mixed and placed at 37 ° C for 60 minutes.
4. Wash the board: the same as before.
5. Add 100 ul of enzyme-labeled antibody working solution per well. The reaction plate was placed at 37 ° C for 30 minutes.
6. Wash the board: the same as before.
7. Add 100 ul of substrate working solution to each well and let it react at 37 ° C for 15 minutes in the dark.
8. Add 100 ul of stop solution to each well and mix.
The absorbance was measured at 450 nm using a microplate reader within 9.30 minutes.